japonicus and adhered to the organs of the body surface. T. japonicus grazing promoted DMS release originating from degradation of DMSP in algal cells. Grazing-activated DMS production decreased because of reduced IR in the presence of microplastics. These results provide new insight into the biogeochemical cycle of sulfur during feeding in copepods exposed to microplastics. Pesticides used in agriculture are some of the most common pollutants in the world. This study aimed to investigate the effects of Organophosphorus Pesticides (OPPs) and Organochlorine Pesticides (OCPs) on the families of farmworkers in the southeast of Iran. In the present case-control study, 141 family members of farmworkers (as the case group) and 59 family members of non-farmworkers (as the controls) were recruited. Serum levels of OCPs such as α-HCH, β-HCH, γ-HCH, 2,4-DDE, 4,4-DDE, 2,4-DDT, and 4,4-DDT were determined. In addition, erythrocyte acetylcholinesterase (AChE) activity, malondialdehyde (MDA), total antioxidant capacity (TAC), protein carbonyl (PC), nitric oxide (NO) serum levels, arylesterase activity of paraoxonase 1 (PON-1), superoxide dismutase (SOD), catalase (CAT), and glutathione peroxidase (GPx) activity were determined in all participants. Furthermore, distance to farmlands, education, crops, type, and the number of consumed fruits were evaluated for each individual separately. Tre higher than expected in the farmworkers' family members. Furthermore, exposure to OCPs and OPPs, apart from the other effects on the body, leads to oxidative stress (OS) that may cause serious diseases in the exposed populations.Contamination of soil with heavy metals seriously harms the growth of crops. Silicon fertilizer is known to promote growth of crops and alleviate heavy metals stresses in vegetables. However, little is known about the effects of silicon fertilizer on pakchoi vegetable growth and soil microbial community in soil contaminated with multiple heavy metals. In order to elucidate this question, current study was designed to analyze the impact of different silicon fertilizer doses on the growth of pakchoi, heavy metals accumulation in pakchoi, and diversity and composition of bacterial community in heavy metals contaminated soil. Results of the study showed that, silicon fertilizer application significantly improved the yield of pakchoi and reduced the content of heavy metals in pakchoi. Moreover, the silicon fertilizer led to the heterogeneity of bacterial community structure in soil. Linear discriminant analysis (LDA) effect size (LEfSe) test showed the change of soil bacterial community structures under the higher silicon fertilizer doses (0.8-3.2%). Similarly, soil bacteria associated with heavy metal resistance and carbon/nitrogen metabolism showed a more active response to medium fertilizer dose (0.8% w/w). In addition, Mantel test and Redundancy analysis (RDA) showed that both the soil bacterial community structures and pakchoi growth were significantly correlated with soil EC, available K and pH. Study suggested that the application of silicon fertilizer provided richer bacteria associated with heavy metal resistance and plant growth, and more favorable soil physicochemical environment for the growth of pakchoi under multiple heavy metal contamination, and the impact was dependent on fertilizing dose.It has been reported that heavy metal contamination can affect litter decomposition and soil properties through its impact on microbial communities. However, it is still unclear whether the expected changes in earthworm activities in responses to heavy metal contamination could affect these properties. Therefore, we quantified earthworm (Eisenia fetida) responses in survival rate to lethal cadmium (Cd) concentrations (0, 50, 100, 150, 200, 250, and 300 mg L-1), and in burrowing ability, physiological characteristics, and feeding rate (on poplar leaf litter) to sub-lethal Cd concentrations (0, 15, 30, and 45 mg kg-1). Finally, sub-lethal influences of Cd on the decomposition rate of poplar leaf litter and on soil properties were investigated in the present of E. fetida. The 12-, 24-, 36-, and 48-h LC50 of Cd for E. fetida were 276.0, 208.6, 192.6, 179.8 mg L-1, respectively. selleck With increasing Cd concentration, malondialdehyde was stimulated, superoxide dismutase first increased and then decreased, while feeding rate, total borrowing length, and maximum burrowing depth consistently decreased. Consequently, leaf-litter decomposition rate and soil nutrient concentrations generally decreased with increasing Cd concentration. Our results indicate that, by affecting earthworm activities, Cd inhibited leaf-litter decomposition and led to the degradation of soil fertility. This study highlights the importance of earthworms in mediating soil functions under heavy metal stress.Trace elements can be toxic when they cannot be easily removed after entering an ecosystem, so a long-term assessment is fundamental to guide ecosystem restoration after catastrophic pollution. In 1998, a pyrite mining accident in Aznalcóllar (south-western Spain) spilled toxic waste over a large area of the Guadiamar river basin, where, after restoration tasks, the Guadiamar Green Corridor was established. Eight years after the mine accident (2005-2006), the ground-dwelling insectivorous lizard Psammodromus algirus registered high trace-element levels within the study area compared to specimens from a nearby unpolluted control site. In 2017, 20 years after the accident, we repeated the sampling for this lizard species and also quantified trace elements in vegetation as well as in arthropod samples in order to identify remnant trace-element accumulation with the aim of assessing the transfer of these elements through the trophic web. We found remnant trace-element contamination in organisms of the polluted site compared to those from the unpolluted site. All trace-element concentrations were higher in arthropods than in plants, suggesting these compounds bioaccumulate through the trophic web. Lizards from the polluted areas had higher As, Cd, and Hg concentrations than did individuals from the unpolluted area. Lizard abundance between sampling periods (2005-06 and 2017) did not vary in unpolluted transects but strongly declined at polluted ones. By contrast, the Normalized Difference Vegetation Index indicated that in the study period, the vegetation was similar at the two sampling sites. These results suggest that, 20 years after the accident, the trace-element pollution could be the cause of a severe demographic decline of the lizard in the polluted area.

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