Then, Au-DNA was captured and alternately hybridized with DNA-modified CdS nanocrystals NCs on the surface of AuNFs with the formation of a super reticulate structure The reticulate structure not only raised another identified ECL signal from CdS NCs but also greatly promoted its ECL intensity from the surface plasmon resonance originating from Au NPs The value of log ECLCdS/ECLluminol and the logarithm of the number of cells exhibit considerable linear relation ranging from 80 to 8 × 105 cells mL-1 with a low detection limit of 20 cells mL-1 S/N = 3 The selectivity and specificity of this dual-potential ECL sensor showed good performance and indicated considerable promise in avoiding false-positive results in detectionHerein, for the first time, we propose that the cleavage activity of DNAzyme is accompanied by the release of hydroxyl ions, which can be used for colorimetric assay Subsequently, we further construct a colorimetric strategy for lipopolysaccharide LPS analysis by using this property Detailly, DNAzyme is split into two fragments separately modified with aldehyde group and hydroxylamine group, which can be linked together through oxime chemistry and the presence of LPS can prevent the formation of oxime bond The formed whole DNAzyme can mediate the release of hydroxyl ions serving for colorimetric signal output Taking LPS as model targets, DNAzyme-based colorimetric assay has been successfully constructed This work not only provides a colorimetric strategy to analyze DNAzyme activity, but also gives a new insight to enrich the versatility of DNAzymes and to enhance their multifunctionalityDesigning a photoelectrochemical PEC immunosensor that can produce dual photocurrent signals which can refer to each other is a great importance but a big challenge In this manuscript, a novel dual photocurrent signals immunosensor was constructed for the detection of α-fetoprotein AFP Unlike the usual method of using two composite materials to provide cathode and anode photocurrent respectively, this work applies only one compound of MIL-101 Cr and CdSe quantum dots QDs Thereinto, we found that the photocurrent polarity of MIL-101Cr would switch by adjusting applied voltage  https//wwwselleckchemcom/products/cy-09html  And then CdSe QDs was introduced by simple ultrasound mixing to boost the dual photocurrent signals Furthermore, in the composite of Mamp;C, the electron transfer path between MIL-101Cr and CdSe QDs may switch between "Z-type" and "Ⅱ-type" by adjusting voltage Benefiting by the dual signals, the proposed sensor can not only perform sensitively quantitative detection of α-fetoprotein AFP, but also can intuitively estimate the accuracy and reliability of the test result by determining whether the corresponding relationship of "cathode photocurrent-analyte concentration-anode photocurrent" is established The linear ranges of the sensing electrodes as cathode and anode are the same, both from 01 to 300 ng mL-1 The limit of detection LOD is 0082 ng mL-1 S/N = 3 when it used as an anode, and the LOD is 0054 ng mL-1 S/N = 3 when it served as cathode Furthermore, this sensor showed acceptable stability, reproducibility, specificity, and feasibility of detecting AFP in human serum, which has broad development prospects in the early clinical diagnosisProgrammed death ligand 1 PD-L1 is a typical immune checkpoint protein, whose up-regulation on the membrane of different tumor cells inhibits the immune response of T cells and leads to the escape of tumor cells In this work, we designed a facile and highly specific surface plasmon resonance SPR biosensor to detect PD-L1 in human plasma based on magnetite nanorods containing ordered mesocages MNOM and silver nanoclusters AgNCs Magneto-optical nanocomplex MNOMAgNCs with superior magneto-optical properties and high signal-to-noise ratio were fabricated to improve the detection sensitivity owing to the high specific surface area of MNOM and excellent localized SPR of AgNCs The PD-L1 Antibody on the surface of gold chip and the PD-L1 aptamer on MNOMAgNCs could realize dual selective recognition of PD-L1, providing the specificity of the sensor and reducing non-specific binding The SPR sensor showed a good linear range of PD-L1 from 10 ng/mL to 300 ng/mL with the detection limit of 329 ng/mL The practical performance of this immunosensing platform had been successfully verified by clinical samples which included healthy donors and cancer patients Based on the analysis, the developed immunosensor provided a new strategy for point-of-care detection of PD-L1 and could be used as clinical companion diagnosis of PD-1/PD-L1 inhibitor therapyThe Body Acceptance by Others Scale-2 BAOS-2 is a 13-item instrument measuring generalised perceptions of body acceptance by others Here, we first demonstrate that a Bahasa Malaysia Malay translation of the BAOS-2 is psychometrically valid in a sample of 1,049 Malaysian adults Study 1 Using exploratory and confirmatory factor analysis, we extracted a unidimensional model of BAOS-2 scores that retained all 13 items BAOS-2 scores had adequate internal consistency and indices of validity convergent, construct, concurrent, and incremental, and were scalar invariant across gender and ethnicity Malaysian Malays vs Chinese Next, we assessed invariance of BAOS-2 scores across samples from Malaysia, the United Kingdom, and the United States total N = 2,575; Study 2 Multi-group confirmatory factor analysis showed that partial scalar invariance was achieved Participants in the interdependent cultural context of Malaysia had significantly higher scores - with small effect sizes - than their counterparts in the independent contexts of the United Kingdom and United States In addition, women had significantly higher scores than men, but the effect size was negligible The present study indicates that the Malay BAOS-2 is a psychometrically valid instrument and presents the first comparison of BAOS-2 scores across interdependent and independent contextsLeptospirosis, a zoonotic disease with worldwide distribution, is caused by spirochetes of the genus Leptospira In dogs, this disease is frequently misdiagnosed Few studies have attempted to associate the detection of Leptospira spp infection with clinicopathological and renal histopathological findings using a multidisciplinary approach The present study isolated and characterized Leptospira spp obtained from naturally infected dogs and described relevant clinical and histopathological findings Blood and urine were collected from 57 dogs with clinical symptomatology suggestive of leptospirosis; 38 cases were confirmed by PCR in urine or by culture or microscopic agglutination testing titers ≥800 A total of 12 strains of pathogenic Leptospira were isolated from the studied dogs seven in blood, four in urine and one in both blood and urine samples All isolates were characterized as Leptospira interrogans serogroup Icterohaemorrhagiae Of the confirmed cases, almost one-third of the animals had been vaccinated