Post-mortem analysis of the cycled cathodes provides insights into the sources of degradation occurring during long-term cycling This work demonstrates a practically viable, synthetic approach combined with doping and coating to achieve improved performance with high-Ni layered oxide materials Furthermore, this work represents the first report of extended cycling of LiNiO2 in pouch full cells with graphite anode and will, therefore, serves as an important benchmark for future research on LiNiO2Protein methylation is one of the most common and important post-translational modifications, and it plays vital roles in epigenetic regulation, signal transduction, and chromatin metabolism However, due to the diversity of methylation forms, slight difference between methylated sites and nonmodified ones, and ultralow abundance, it is extraordinarily challenging to capture and separate methylated peptides from biological samples Here, we introduce a simple and highly efficient method to separate methylated and nonmethylated peptides using 18-crown-6 as a mobile phase additive in high-performance liquid chromatography Selective complexation between lysine and 18-crown-6 remarkably increases the retention of the peptides on a C18 stationary phase, leading to an excellent baseline separation between the lysine methylated and nonmethylated peptides A possible binding mechanism is verified by nuclear magnetic resonance titration, biolayer interferometry technology, and quantum chemistry calculation Through establishment of a simple enrichment methodology, a good selectivity is achieved and four methylated peptides with greatly improved signal-to-noise S/N ratios are successfully separated from a complex peptide sample containing 10-fold bovine serum albumin tryptic digests By selecting rLys N as an enzyme to digest histone, methylation information in the histone could be well identified based on our enrichment method This study will open an avenue and provide a novel insight for selective enrichment of lysine methylated peptides in post-translational modification proteomicsMetal-silicon nanowire array photoelectrodes provide a promising architecture for water-splitting because they can afford high catalyst loading and decouple charge separation from the light absorption process To further improve and understand these hybrid nanowire photoelectrodes, control of the catalyst amount and location within the wire array is required Such a level of control is currently synthetically challenging to achieve  https//wwwselleckchemcom/products/10-dab-10-deacetylbaccatinhtml  Here, we report the synthesis of cm2-sized hybrid silicon nanowire arrays with electrocatalytically active Ni-Mo and Pt patches placed at defined vertical locations within the individual nanowires Our method is based on a modified three-dimensional electrochemical axial lithography 3DEAL, which combines metal-assisted chemical etching MACE to produce Si nanowires with spatially defined SiO2 protection layers to selectively cover and uncover specific areas within the nanowire arrays This spatioselective SiO2 passivation yields nanowire arrays with well-defined exposed Si surfaces, with feature sizes down to 100 nm in the axial direction Subsequent electrodeposition directs the growth of the metal catalysts at the exposed silicon surfaces As a proof of concept, we report photoelectrocatalytic activity of the deposited catalysts for the hydrogen evolution reaction on p-type Si nanowire photocathodes This demonstrates the functionality of these hybrid metal/Si nanowire arrays patterned via 3DEAL, which paves the way for investigations of the influence of three-dimensional geometrical parameters on the conversion efficiency of nanostructured photoelectrodes interfaced with metal catalysts"Quenchbody Q-body" is a quench-based fluorescent biosensor labeled with a fluorescent dye near the antigen-binding site of an antibody Q-bodies can detect a range of target molecules quickly by simply mixing with a sample However, the development of Q-bodies using VHH-nanobodies derived from camelid heavy-chain antibodies has not been reported despite their favorable characteristics Here, we report a "mini Q-body" that can detect the chemotherapy agent methotrexate MTX by using anti-MTX nanobody Three kinds of constructs each encoding an N-terminal Cys-tag and anti-MTX VHH gene with a different length of linker GGGS n n = 0, 2, and 4 between them were prepared followed by the expression in Escherichia coli and labeling with several dye maleimides When the fluorescence intensities in the presence of varied MTX concentrations were measured, TAMRA-labeled nanobodies showed a higher response than ATTO520- or R6G-labeled ones Especially, TAMRA C6-labeled mini Q-body with no linker showed the highest response of ∼6-fold and a low detection limit of 056 nM When each Trp residue in the mini Q-body was mutated to address the quenching mechanism, the major role of Trp34 at CDR1 in quenching was revealed Furthermore, the mini Q-body could detect MTX in 50 human serum with a low detection limit of 172 nM, showing its applicability to therapeutic drug monitoring This study is expected to become the basis of the construction of highly responsive mini Q-bodies for sensitive detection of many molecules from small haptens to larger proteins, which will lead to broader applications such as point-of-care testsBipolar membranes BPMs have the potential to become critical components in electrochemical devices for a variety of electrolysis and electrosynthesis applications Because they can operate under large pH gradients, BPMs enable favorable environments for electrocatalysis at the individual electrodes Critical to the implementation of BPMs in these devices is understanding the kinetics of water dissociation that occurs within the BPM as well as the co- and counter-ion crossover through the BPM, which both present significant obstacles to developing efficient and stable BPM-electrolyzers In this study, a continuum model of multi-ion transport in a BPM is developed and fit to experimental data Specifically, concentration profiles are determined for all ionic species, and the importance of a water-dissociation catalyst is demonstrated The model describes internal concentration polarization and co- and counter-ion crossover in BPMs, determining the mode of transport for ions within the BPM and revealing the significance of salt-ion crossover when operated with pH gradients relevant to electrolysis and electrosynthesis