Confirmed to be a new type of food resource, quail egg can provide humans with high-quality protein and offer various nutrients that can promote growth and development Post-translational modification of proteins can regulate their molecular structures and physiological functions However, the understanding and related research of quail egg holoproteins and post-translationally modified proteins is not yet sufficient This study provides an in-depth analysis of quail egg proteins using an omics strategy A total of 175 proteins, 109 N-glycoproteins 293 N-glycosylation sites and 23 phosphoproteins 84 phosphorylation sites were identified Motif analysis showed that N-glycosylation sites of quail eggs were classical sites The main characteristic sequence of the phosphorylation site is "S-X-E" 77 Functional analysis indicated that quail egg proteins, modified proteins were enriched in the regulation of enzyme activity These results have significant reference value for understanding the structure, function of quail eggs, explaining the physicochemical reaction during the storageCloned pigs generated by the somatic cell transfer nuclear SCNT technique are highly valuable for agriculture, biomedicine, and life sciences However, the neonatal mortality rate of cloned pigs is very high The reasons causing the massive loss of cloned pigs during their neonatal ages are unclear In the present study, we found that the neonatal death of cloned pigs was associated with aberrant purine metabolism, impaired renal morphology and function, and decreased hepatic Hprt1 expression The downregulation of Hprt1, a key purine metabolism regulation gene, in the liver was responsible for the elevation of an important purine metabolite, uric acid, in the serum, causing abnormalities in kidney morphology and function and leading to death of neonatal cloned pigs This study provided insights into the pathophysiological mechanisms underlying the neonatal death of clone pigs, and results will help improve their survival rateAdipose dysfunction and inflammation with or without hepatic defects underlie metabolic obesity Glutamine GLU improves glucoregulation and metabolic indices but its effects on adipose function and hepatic lipid deposition in estrogen-progestin oral contraceptive EPOC users are unknown  https//wwwselleckchemcom/products/arry-380-ont-380html  Therefore, we hypothesized that GLUT supplementation would protect against adipose dysfunction and excess hepatic lipid influx and deposition in EPOC-treated animals by suppressing adenosine deaminase/xanthine oxidase ADA/XO activity and improving glucose-6-phosphate dehydrogenase G6PD-dependent antioxidant defense Female Wistar rats weighing 150-180 g were allotted into control, GLUT, EPOC and EPOC + GLUT groups six rats/group The groups received vehicle distilled water, po, GLUT 1 g/kg, EPOC containing 10 µg ethinylestradiol plus 50 µg levonorgestrel and EPOC plus GLUT, respectively, daily for 8 weeks Results showed that the administration of EPOC caused glucose dysregulation and increased triglyceride-glucose index and visceral adiposity, but the body weight and liver weight were not affected However, EPOC significantly decreased adipose lipid, G6PD and glutathione and increased glycogen synthesis, ADA, XO, uric acid, lipid peroxidation, lactate production and gamma-glutamyl transferase activity GGT On the other hand, EPOC increased hepatic lipid, ADA, XO, uric acid, lipid peroxidation and lactate production and decreased glycogen synthesis, G6PD and glutathione Nevertheless, supplementation with glutamine attenuated these alterations Collectively, the present results indicate that EPOC causes metabolically induced obesity which is associated with adipose dysfunction and hepatic metabolic disturbance The findings also suggest that glutamine confers metabo-protection with corresponding improvement in adipose and hepatic metabolic function by suppression of ADA/XO activity and enhancement of G6PD-dependent antioxidant defenseCinnamaldehyde CA is an essential component of cinnamon Cinnamomum cassia Presland, which is often used as a flavoring condiment in beverages, pastries, perfumes, etc Cinnamon is also used as herbal medicine in China and Southeast Asia to treat rheumatoid arthritis However, the molecular mechanism is unclear In this study, we aim to investigate its anti-inflammatory effects against Rheumatoid arthritis RA using activated macrophages Raw2467 in vitro and adjuvant arthritis rats AA in vivo The results demonstrated that CA significantly reduced synovial inflammation in AA rats, possibly due to suppression of the expressions of pro-inflammatory cytokines, especially the IL-1β Further investigation found that CA also suppressed the activity of HIF-1α by inhibiting the accumulation of succinate in cytoplasm As we know, the reduction of HIF-1α nucleation slows down IL-1β production, because HIF-1α activates the expression of NLRP3, which is involved in the assembly of inflammasome and processing of IL-1β In addition, CA also inhibited the expression of the succinate receptor GPR91, which in turn inhibited the activation of HIF-1α In conclusions, our results suggested that CA might be a potential therapeutic compound to relieve rheumatoid arthritis progress by suppressing IL-1β through modulating succinate/HIF-1α axis and inhibition of NLRP3Background and aims Cardiovascular disease CVD begins in youth, and is exacerbated by obesity and metabolic syndrome Apolipoprotein ApoB-remnant cholesterol is considered a primary contributor to CVD risk Fasting plasma apoB48 can be used as a biomarker of intestinal remnant cholesterol as well as postprandial dyslipidemia In adults, elevated fasting plasma apoB48 strongly associates with cardiometabolic risk factors and obesity, whereas in adolescents there is limited data The aim of this study was to measure fasting plasma apoB48 and determine the relationship with cardiometabolic risk factors in adolescents Methods This is a cross-sectional study of fasting plasma apoB48 from the Western Australian Pregnancy Cohort Raine Study Subjects were adolescent males and females aged 17 years with complete fasting plasma apoB48, biochemical, and anthropometry data n = 1045 The relationship between fasting plasma apoB48 and other cardiometabolic risk factors was determined The high-risk metabolic cluster variable was defined using elevated BMI, HOMA-IR, fasting plasma triglycerides, and systolic blood pressure Results Fasting plasma apoB48 was significantly higher in male 1528 ± 295 μg/mL compared to female 1245 ± 243 μg/mL adolescents p = 00003, and was increased by 21 360 μg/mL; p = 00000 in the high-risk metabolic cluster group and more pronounced in males 31, 615 μg/mL; p = 00000 Fasting plasma apoB48 was positively associated with fasting plasma triglycerides, total-cholesterol but not LDL-C, insulin, leptin, HOMA-IR, and the anthropometric parameters, waist-circumference and skinfold-thickness Fasting plasma apoB48 was inversely associated with fasting plasma HDL-C, and adiponectin Conclusions Plasma apoB48 remnant lipoproteins associate with cardiometabolic risk factors in adolescents and provide support for the screening of remnant cholesterol in youthObjective Single dose administration of methotrexate MTX is considered the first line of treatment in selected patients with an ectopic pregnancy EP However, data regarding MTX efficacy among obese patients is limited We sought to investigate the efficacy of MTX single dose regimen among obese patients MATERIAL AND METHODS A retrospective cohort study conducted at a gynecology department in a tertiary teaching hospital, between January 2010 and December 2018, including women diagnosed with an EP and treated by a single-dose regimen of MTX We compared success rate and gestation characteristics between obese and non-obese women Results Overall, 195 women were treated with single-dose intramuscular MTX for EP during the study period Of those, 31 women 159 were obese BMI ≥ 30 kg/m2 and the rest 164 841 were of normal body weight Median MTX dosage for the obese group was 95 milligrams IQR 91-104 vs 83 milligrams IQR 78-87 for the non-obese group link2 Treatment success rate of the overall cohort was 666 130/195 and treatment success rate of single-dose MTX was comparable between the obese and non-obese groups 645 vs 670, p = 078 Obese patients were older as compared to non-obese median age 33 vs 29, p = 003 In multivariate logistic regression analysis, percentage hCG change from day 1 to day 4 was the only factor associated with treatment success aOR 102; 95CI 101, 104, p less then 0001 Conclusion Single-dose MTX treatment among obese patients diagnosed with ectopic pregnancy led to similar success rates as compared to non-obese patientsThe safety of diclofenac DIC use in clinical practice has been questioned because of adverse cardiovascular effects Previous studies have indicated that DIC cause mitochondrial dysfunction and oxidative stress in heart mitochondria The aim of this study was to investigate the protective effect of calcitriol against the mitochondrial toxicity potency of diclofenac in heart rat mitochondria For this purpose, rat heart mitochondria were isolated with mechanical lysis and differential centrifugation Then isolated mitochondria were pretreated with 3 different concentrations of calcitriol 25, 5 and 10 µM for 5 min at 37°C, after which DIC 10 µg/ml was added to promote deleterious effects on mitochondria During 1 hour of incubation, using by flow cytometry and biochemical evaluations, the parameters of mitochondrial toxicity were evaluated link3 Our results showed that DIC 10 µg/ml caused a significant decrease in succinate dehydrogenase SDH activity, mitochondrial membrane potential MMP collapse, and mitochondrial swelling, and a significant increase in reactive oxygen species ROS formation, lipid peroxidation LP and oxidative stress Also, our results revealed that co-administration of calcitriol 5 and 10 µM with diclofenac markedly ameliorates the mitochondrial toxicity effects in rat hart mitochondria In this study, we showed that DIC impairs mitochondrial function and induces mitochondrial toxicity in rat heart isolated mitochondria, which were ameliorated by calcitriol These findings suggest that calcitriol may be a preventive/therapeutic strategy for cardiotoxicity complications caused by DICThe use of in vitro embryo production in the horse is increasing in clinical and research settings, however, protocols are yet to be optimised Notably, the two most commonly used base media for in vitro maturation IVM supply glucose at markedly different concentrations physiological 56 mM, M199 or supraphysiological 17 mM, DMEM/F-12 Exposure to high glucose has detrimental effects on oocytes and early embryos in many mammalian species, but the impact has not yet been examined in the horse To address this, we compared the energy metabolism of equine COCs matured in M199-based maturation medium containing either 56 or 17 mM glucose, as well as expression of key genes in oocytes and cumulus cells Oocytes were fertilised by ICSI and cultured Analysis of spent medium revealed that COC glucose consumption and production of lactate and pyruvate were similar between treatments However, the glycolytic index was decreased at 17 mM and analysis of mitochondrial function of COCs revealed that IVM in 17 mM glucose was associated with decreased ATP-coupled respiration and increased non-mitochondrial respiration compared to that for 5