The development of a simple, sensitive, and effective method for the analysis of circulating tumor cells CTCs is essential for cancer diagnosis and metastasis prediction In this work, we have proposed an enzyme-free electrochemical method for specific capture, sensitive quantification, and efficient release of CTCs To achieve this, the specific interaction between CTCs and the corresponding aptamer designed to be located in the identification probe IP will unfold the hairpin structure of IP Consequently, IP will initiate a hybridization reaction to produce a duplex, which will further trigger the hybridization chain reaction HCR process to form a composite product of CTCs and double-stranded DNA polymers Therefore, a significantly amplified signal readout can be obtained Moreover, the composite product can be brought to the electrode surface by tetrahedral DNA nanostructures to achieve the purpose of capturing and quantifying CTCs More significantly, these captured CTCs can be controlled released without compromising cell viability via a simple strand displacement reaction Taking the breast cancer cell MCF-7 as a representative, the newly developed approach led to an ultralow detection limit of 3 cells mL-1, which is superior to several studies previously reported The current method has also been demonstrated to analyze CTCs in human whole blood and hence revealed a great potential in the futureThe generalization of related asymmetric processes in organocatalyzed reactions is an ongoing challenge due to subtle, noncovalent interactions that drive selectivity The lack of transferability is often met with a largely empirical approach to optimizing catalyst structure and reaction conditions This has led to the development of diverse structural catalyst motifs and inspired unique design principles in this field Bifunctional hydrogen bond donor HBD catalysis exemplifies this in which a broad collection of enantioselective transformations has been successfully developed Herein, we describe the use of data science methods to connect catalyst and substrate structural features of an array of reported enantioselective bifunctional HBD catalysis through an iterative statistical modeling process The computational parameters used to build the correlations are mechanism-specific based on the proposed transition states, which allows for analysis into the noncovalent interactions responsible for asymmetric induction The resulting statistical models also allow for extrapolation to out-of-sample examples to provide a prediction platform that can be used for future applications of bifunctional hydrogen bond donor catalysis Finally, this multireaction workflow presents an opportunity to build statistical models unifying various modes of activation relevant to asymmetric organocatalysisThis work describes the development of easy-to-prepare cobalt nanoparticles NPs in solution as promising alternative catalysts for alkene hydrosilylation with the industrially relevant tertiary silane 1,1,1,3,5,5,5-heptamethyltrisiloxane MDHM The Co NPs demonstrated high activity when used at 30 °C for 35-7 h in toluene, with catalyst loadings 005-02 mol , without additives Under these mild conditions, a set of terminal alkenes were found to react with MDHM, yielding exclusively the anti-Markovnikov product in up to 99 yields Additionally, we demonstrated the possibility of using UV irradiation to further activate these cobalt NPs not only to enhance their catalytic performances but also to promote tandem isomerization-hydrosilylation reactions using internal alkenes, among them unsaturated fatty ester methyl oleate, to produce linear products in up to quantitative yieldsA high-performance holographic recording medium was developed based on a unique combination of photoinitiated thiol-ene click chemistry and functional, linear polymers used as binders Allyl reactive sites were incorporated along the backbone of the linear polymer binder to enable facile film casting and to facilitate cross-linking by photopolymerization of the thiol-ene monomers that also serve as the writing monomers in this distinctive approach to holographic materials The allyl content and the ratio of the linear polymer to the writing monomers were varied to maximize and control the refractive index contrast A blade-coating-based film preparation method was developed to form films from the mixture of linear polymer and the thiol-ene monomers This approach results in a holographic material with a peak to mean index contrast Δn that reaches 004 The refractive index contrast was stable for at least two weeks  https//wwwselleckchemcom/products/GDC-0449html  Haze in holograms with a high writing monomer loading was significantly reduced when a higher allyl content was incorporated into the binder, resulting in the lowest haze around 02 Finally, the media exhibit high resolution as demonstrated by the ability to record reflection holograms with 140 nm pitch and diffraction efficiency in excess of 90The aim of this study was to investigate the detailed mechanisms of hepatotoxicity induced by cadmium telluride quantum dots CdTe-QDs in BALB/c mice after intravenous injection The study investigated oxidative stress, apoptosis, and effects on mitochondria as potential mechanistic events to elucidate the observed hepatotoxicity Oxidative stress in the liver, induced by CdTe-QD exposure, was demonstrated by depletion of total glutathione, an increase in superoxide dismutase activity, and changes in the gene expression of several oxidative stress-related biomarkers Furthermore, CdTe-QD treatment led to apoptosis in the liver via both intrinsic and extrinsic apoptotic pathways Effects on mitochondria were evidenced by the enlargement and increase in the number of mitochondria in hepatocytes of treated mice CdTe-QDs also caused changes in the levels and gene expression of electron transport chain enzymes, depletion of ATP, and an increase in the level of the peroxisome proliferator-activated receptor gamma coactivator 1-alpha PGC-1α, a regulator of mitochondrial biogenesis The findings from this study suggest that CdTe-QDs-induced hepatotoxicity might have originated from mitochondrial effects which resulted in oxidative stress and apoptosis in the liver cells This study provides insight into the biological effects of CdT-QDs at the tissue level and the detailed mechanisms of their toxicity in animals The study also provides important data for bridging the gap between in vitro and in vivo testing and risk assessment of these NPs