In reconstructing a complex defect associated with infection, using muscle flaps to fill the dead space with well vascularized tissue is considered to be appropriate In our case, we chose a rectus femoris muscle flap, which has advantages in volume and versatility of transposition owing to long vascular pedicle and requires no microsurgical vascular anastomosis  https//wwwselleckchemcom/products/Sunitinib-Malate-Sutenthtml  As a result, the preoperative activity was maintained, the infection was treated, and a good course was obtainedTwo flaps, namely the free vascularized iliac bone graft supplied by the deep circumflex iliac artery DCIA and the superficial circumflex iliac artery perforator flap supplied by the superficial circumflex iliac artery SCIA, can be individually harvested from a single surgical field We report two cases treated by these free flaps for severe hand injury with large skin defect and osteomyelitis Sequential chimeric flaps were anastomosed between the ascending branch of the DCIA and the SCIA The advantage of this method is more freedom in the flap insetting for complex tissue defects For this reason, this method is also excellent for cosmetic appearance Furthermore, donor site morbidity can be minimized because the flaps are harvested from the same site
  The role of point-of-care testing POCT out of hospital, especially in home care and ambulatory care settings, is an issue meriting further research We reviewed studies reporting cardiovascular events as a result of the implementation of B-type natriuretic peptide or N-terminal pro B-type natriuretic peptide POCT BNP/NT-proBNP POCT for heart disease patients in the settings
 
  Articles were searched via a PubMed engine until May 30, 2020
 
  In total, six studies were selected Three studies involving ambulatory care used the POCT to refer patients with suspected heart diseases to a specialist The other three used the tests in home care to monitor patients with heart failure In ambulatory care, the randomized controlled trials, in which referrals were made to a specialist, showed that the group using POCT had significantly fewer cardiovascular outcomes, such as hospitalizations and deaths, than the non-use group In home care, adverse outcomes were predicted from changes in BNP levels
 
  In most studies, the use of BNP/NT-proBNP POCT in home care and ambulatory care settings demonstrated favorable results regarding the cardiovascular outcomes The utility of POCT in the settings is suggested, while more investigations are required
 In most studies, the use of BNP/NT-proBNP POCT in home care and ambulatory care settings demonstrated favorable results regarding the cardiovascular outcomes The utility of POCT in the settings is suggested, while more investigations are required
  Increasing HIFs in malignant melanoma, the highly aggressive skin tumour, results in the stimulation of invasiveness Increased HIF-1α fallouts in inhibition of the activity of some mitochondrial enzymes and leads to preference of cytosol energetic metabolism Increase of aerobic glycolysis is reflected in an increase of free NADH Warburg effect and develops the malignant melanomaOur goal was to find a link between hypoxia, or hypoxia mimicking factors and the stage of malignant melanoma Furthermore, we focused on the finding of the experimental parameter which could monitor melanoma patients
 
  We targeted HIF-1α gene expression and VDR rs2107301 gene polymorphism by PCR analysis We detected the level of NADH in blood plasma by fluorescence spectroscopy excitation and emission spectra
 
  Analysis of the obtained data from patient samples has shown an increase in HIF-1α which correlates with the disease stage Investigation VDR rs2107301 polymorphism of patient samples does not show any significant changes in single nucleotide polymorphism, and the low vitamin D level in blood is not a result of VDR mutation in mitochondria NADH levels vary under hypoxic and pseudohypoxic conditions and refer to the cancer stage
 
  The apparent mismatch between HIF-1α expression and NADH fluorescence has become the basis for the design of an algorithm for monitoring malignant melanoma based on the sensing of NADH fluorescence and the determination of HIF-1α
 The apparent mismatch between HIF-1α expression and NADH fluorescence has become the basis for the design of an algorithm for monitoring malignant melanoma based on the sensing of NADH fluorescence and the determination of HIF-1α
  The prevalence of familial hypercholesterolemia FH among Japanese populations is still unclear In addition, no prior data exist regarding the self-awareness Accordingly, we aimed to investigate the prevalence, self-awareness, and LDL-C of patients with highly suspected as FH using data obtained in a community-based medical checkups
 
  This study included 52,276 subjects 18,588 men, 356 aged ≥40 years who underwent the Japanese specific health checkup in Kanazawa City during 2018 We assessed the self-awareness of dyslipidemia and the age as well as the prevalence of patients with highly suspected as FH whose naïve LDL-C levels were ≥250​mg/dl Naïve LDL-C levels were estimated by the adjustment LDL-C/07 for those on lipid-lowering medication We divided subjects into 3 groups based on their naïve LDL cholesterol level ≥250​mg/dl, 140-249, and ≤139​mg/dl
 
  We identified 262 05 individuals highly suspected as FH whose naïve LDL-C levels were ≥250​mg/dl Most of them 234 among 262, 89 were under lipid-lowering medication; however, the self-awareness as dyslipidemia was not quite high 200 among 262, 76, and their mean LDL-C level under lipid-lowering medication was 203​±​35​mg/dl Interestingly, the age of acknowledgement of dyslipidemia among the patients with highly suspected as FH was significantly younger than those in other categories 58 vs 60/62​yrs, respectively, p​lt;​005 for both
 
  The prevalence of patients highly suspected as FH was around 1 in 200, and their self-awareness as well as control were not still good enough among Japanese general populations
 The prevalence of patients highly suspected as FH was around 1 in 200, and their self-awareness as well as control were not still good enough among Japanese general populations