Decreased work ability t2 was associated with more cognitive impairment, higher effort coping with the disease, comorbidities, sick leave gt; 6 months since diagnosis, and having children adj R
  = 48 Cognitive impairments occurred in approximately every fifth patient at both surveys
 
  Achieving maximum work ability is a major challenge for AYAs Our results show the need for multimodal cancer survivorship and rehabilitation programmes with a special focus on improving cognitive and psychosocial functioning
 
  AYAs with cancer should receive targeted occupational counselling early in the course of the disease
 AYAs with cancer should receive targeted occupational counselling early in the course of the disease
  We aimed to explore the real-world clinical application value and challenges of metagenomic next-generation sequencing mNGS for pulmonary infection diagnosis
 
  We retrospectively reviewed the results of mNGS and conventional tests from 140 hospitalized patients with suspected pulmonary infections from January 2019 to December 2020 The sample types included bronchoalveolar lavage fluid, lung tissue by transbronchial lung biopsy, pleural effusion, blood, and bronchial sputum Apart from the mNGS reports that our patients received, an extra comprehensive and thorough literature search was conducted
 
  Significant differences were noticed in the positive detection rates of pathogens between mNGS and conventional diagnostic testing 115/140, 8214 vs 50/140, 3571, P lt; 005 The percentage of mNGS-positive patients was significantly higher than that of conventional testing-positive patients with regard to bacterial detection P lt; 001, but no significant differences were found with regard to fungaled to be refined
 mNGS is a valuable tool for the detection of pulmonary infections, especially mixed pulmonary infections The most common combinations we found were bacterial-fungal coinfection and bacterial-bacterial coinfection Still, there are many challenges in the clinical application of mNGS in the diagnosis of pulmonary infections There is still a lot of work to be done in interpreting the mNGS reports, because both clinical judgment and literature analysis strategy need to be refinedPertussis is a highly contagious disease of the respiratory tract caused by Bordetella pertussis Although the burden of pertussis is highest in children, available data suggests that pertussis in the elderly and those with underlying chronic conditions or illnesses can result in significant morbidity, mortality and costs We undertook a comprehensive review to assess the association between pertussis and chronic conditions/illnesses A search was undertaken on 17 June 2019 across EMBASE, Medline and BIOSIS Citations were limited to those in English, in humans and published since 1 January 1990  https//wwwselleckchemcom/products/3-methyladeninehtml  There were 1179 papers identified with an additional 70 identified through a review of the reference lists Of these, 34 met the inclusion criteria Papers included were categorised in groups, those which reported associations between prior pertussis and subsequent chronic conditions or illnesses; a link between chronic conditions/illnesses and subsequent risk of pertussis; and those which reported on the effect of tirm or disprove these associations, and to characterise the pathophysiological mechanisms behind the potential associations with pertussisMesenchymal stem cells MSCs are self-renewing, multi-potent heterogeneous stem cells that display strong tissue protective and restorative properties by differentiating into cells of the mesodermal lineages In addition to multi-lineage differentiation capacity, MSCs play important roles in regulating immune responses, inflammation, and tissue regeneration MSCs play a role in the outcome of the pathogenesis of several infectious diseases A unique subset of MSCs accumulates in secondary lymphoid organs during malaria disease progression These MSCs counteract the capacity of malaria parasites to subvert activating co-stimulatory molecules and to regulate expression of negative co-stimulatory molecules on T lymphocytes Consequently, MSCs have the capacity to restore the functions of CD34+ haematopoietic cells and CD4+ and CD8+ T cells during malaria infection These observations suggest that cell-based therapeutics for intervention in malaria may be useful in achieving sterile clearance and preventing disease reactivation In addition, MSCs provide host protection against malaria by reprogramming erythropoiesis through accelerated formation of colony-forming-units-erythroid CFU-E cells in the bone marrow These findings suggest that MSCs are positive regulators of erythropoiesis, making them attractive targets for treatment of malarial anemia MSC-based therapies, unlike anti-malarial drugs, display therapeutic effects by targeting a large variety of cellular processes rather than a single pathway In the present review we focus on these recent research findings and discuss clinical applications of MSC-based therapies for malaria
  Antenatal care ANC, delivery by skilled birth attendants, and postnatal care PNC are critical components of maternal health services for reducing maternal mortality The study aimed to compare the utilization of maternal health services in the two most recent rounds of Ethiopia Demographic and Health Surveys EDHS and identify the factors influencing the utilization of these services using the 2016 EDHS
 
  Two rounds of EDHS data in 2011 and 2016 were used to estimate the proportion of women who had ANC, delivered by skilled birth attendants, and had a postnatal checkup and other characteristics of the surveyed population The most recent round of data-the 2016 EDHS-was used to examine the socio-cultural and reproductive health factors associated with the three maternal health services utilization Chi-square tests and multivariate logistic regression analyses with adjusted Odds Ratios AOR were conducted using Stata 150
 
  The use of ANC services and skilled birth attendants increased significantly rural and urban areas, and the need of addressing the social, economic, and physical barriers that prevent women from using these services Further, programs should be targeted at promoting the use of professional birth and postnatal services in EthiopiaGuillain-Barré syndrome GBS is an autoimmune disorder of the peripheral nervous system that typically develops within 4 weeks after infection In addition to conventional infectious diseases with which we are familiar, emerging infectious diseases, such as Zika virus infection and coronavirus disease 2019 COVID-19 caused by severe acute respiratory syndrome coronavirus 2 SARS-CoV-2, have also been suggested to be associated with GBS GBS is mainly categorized into a demyelinating subtype known as acute inflammatory demyelinating polyneuropathy AIDP and an axonal subtype known as acute motor axonal neuropathy AMAN link2 Most patients who develop GBS after Zika virus infection or COVID-19 have AIDP The concept of molecular mimicry between pathogens and human peripheral nerve components was established through studies of AMAN with anti-ganglioside GM1 antibodies occurring after Campylobacter jejuni infection Although such mimicry between specific pathogens and myelin or Schwann cell components has not beeas been demonstratedHerein, we reported a novel series of α-aminophosphonates derivatives IVa-m bearing an important pharmacophore coumarylthiazole moiety All the new compounds have been synthesized via Kabachnik-Fields reaction under ultrasonic irradiation The products were obtained in good yield with a simple workup and were confirmed using various spectroscopic methods All these compounds IVa-m were screened for their in vitro for antimicrobial activity against thirteen Gram-negative bacteria and five Gram-positive bacteria and Candida albicans strains The results showed that all the synthesized compounds exhibited moderate antibacterial activities against both references and multidrug-resistant and antifungal strains The compound IVe showed the highest activities against all pathogens of the tested microbial strains with MIC of 0125 μg/mL link3 The compounds IVh, IVf, IVb, and IVd exhibited moderate and promising activities with MIC of 0125 μg/mL Structure-activity relationship revealed that inhibitory activity of the synthesized compounds is related to the type of the substituted group on phenyl rings, and these results showed that the electron-donating groups at ortho and para positions have a high relationship increasing antimicrobial activities than the electron-withdrawing groups These results confirm that coumarylthiazole α-aminophosphonates compounds can be potential antimicrobial drugs candidateThe SARS-CoV-2 helicase Nsp13 is a promising target for developing anti-COVID drugs In the present study, we have identified potential natural product inhibitors of SARS-CoV-2 Nsp13 targeting the ATP-binding site using molecular docking and molecular dynamics MD simulations MD simulation of the prepared crystal structure of SARS-CoV-2 Nsp13 was performed to generate an ensemble of structures of helicase Nsp13 capturing the conformational diversity of the ATP-binding site A natural product library of more than 14,000 phytochemicals from Indian medicinal plants was used to perform virtual screening against the ensemble of Nsp13 structures Subsequently, a two-stage filter, first based on protein-ligand docking binding energy value and second based on protein residues in the ligand-binding site and non-covalent interactions between the protein residues and the ligand in the best-docked pose, was used to identify 368 phytochemicals as potential inhibitors of SARS-CoV-2 helicase Nsp13 MD simulations of the top inhibitors complexed with protein were performed to confirm stable binding, and to compute MM-PBSA based binding energy From among the 368 potential phytochemical inhibitors, the top identified potential inhibitors of SARS-CoV-2 helicase Nsp13 namely, Picrasidine M, +-Epiexcelsin, Isorhoeadine, Euphorbetin and Picrasidine N, can be taken up initially for experimental studies
  We identified a patient with a novel heterozygous variant fibrinogen, γpC352R Niigata II; N-II, who had a bleeding episode and failed infertility treatment and was suspected to have hypodysfibrinogenemia based on low and discordant fibrinogen levels functional assay 033g/L, immunological assay 091g/L We analyzed the mechanism of this rare phenotype of a congenital fibrinogen disorder
 
  Patient plasma fibrinogen was purified and protein characterization and thrombin-catalyzed fibrin polymerization performed Recombinant fibrinogen-producing Chinese hamster ovary CHO cells were established and the assembly and secretion of variant fibrinogen analyzed by ELISA and western blotting
 
  Purified N-II plasma fibrinogen had a small lower molecular weight band below the normal γ-chain and slightly reduced fibrin polymerization A limited proportion of pC352R fibrinogen was secreted into the culture medium of established CHO cell lines, but the γ-chain of pC352R was synthesized and variant fibrinogen was assembled inside the cells