Iron incorporation is essential for the record activity of NiFe-(oxy)hydroxides to oxygen evolution reaction (OER), but the details of how Fe affects catalysis remain under active investigation. In this work, we present a double thin-layer strategy for finding unique and solid evidence for the role of Fe in the OER mechanism. A thin-layer catalyst of a few nanometers of thickness was deposited on a Ni substrate and a thin-layer electrolyte of 0.1 mm thickness was created using a thin-layer spectroelectrochemical cell. The OER activity, the catalyst composition, and the electrolyte species were investigated together as a function of the Fe deposition time. The results show that trace Fe incorporation favors the formation of β-NiOOH in the thin-layer catalyst and effectively suppresses the dissolution of NiOOH into the electrolyte. The results of double-potential step chronoabsorptometry and cyclic voltabsorptometry demonstrate the potential-dependent formation of a Ni3+ intermediate in the electrolyte and, more importantly, the dissolution suppression effect due to Fe incorporation. These findings link the role of Fe in OER catalysis to the increased insolubility of Ni3+ active sites and highlight the importance of paying close attention to the active-site stability of an electrocatalyst impaired by the electrolyte at a reaction potential.Over 20 million ventral hernia repairs are performed worldwide annually and only a minority ( less then 10%) of cases are not mesh-based. However, even polypropylene (PP), endorsed as the "gold standard" of all prosthetic materials used in this field, is still subject to many complications caused by the foreign body reaction (FBR). Here, we describe the buildup of dopamine-mediated zwitterionic poly(sulfobetaine methacrylate) (PSBMA) coatings to inhibit nonspecific protein adsorption. Based on the universal adhesive ability of polydopamine (PDA), PSBMA has been coated on the PP mesh surface via two strategies sequential deposition (PSBMA-PDA-PP) and co-deposition (PSBMA@PDA-PP). The presence of PSBMA shows great contribution to obviously decreased hydrophobicity of the PP surface (WCAco = 36.3° and WCAseq = 30.7°) as well as improved protein resistance (Reductionco = 74% and Reductionseq = 82%). Notably, as the intermedia between PP and PSBMA, PDA can endow the PP mesh with antioxidant activity, further featuring synergistic anti-inflammation therapeutic effect when coupled with PSBMA. With almost equal surface content of PSBMA, PSBMA-PDA-PP exhibited a more superior ability against macrophage adhesion and proliferation and showed more significantly decreased releases of TNF-α and IL-6 (p less then 0.05) than those of PSBMA@PDA-PP, fundamentally attributed to its more neutral surface potential and the protection for polyphenols of PDA from oxidation with PSBMA as the outer layer. Furthermore, the coating layers demonstrated good stability and no sacrifice of the pristine mechanical property. PF-07265807 The proposed dopamine-mediated PSBMA coatings possess high potential in biomedical implant areas for attenuating the FBR.In contrast to microdroplet condensation with high contact angles, the one with low contact angles remains unclear. In this study, we investigated dynamics of microdroplet condensation of low-surface-tension liquids on two flat substrate surfaces by using reflection interference confocal microscopy. Spontaneous migration toward relatively larger droplets was first observed for the microdroplets nucleated on the hydrophilic quartz surface. The moving microdroplets showed a contact angle hysteresis of ∼0.5°, which is much lower than the values observed on typical flat substrates and is within the range observed on slippery lubricant-infused porous surfaces. Because the microdroplets on the hydrophobic polydimethylsiloxane surface did not move, we concluded that the ultrathin precursor film is formed only on the hydrophilic surface, which reduces a resistive force to migration. Also, reduced size of droplets promotes the thermocapillary motion, which is induced by a gradient in local temperature inside a small microdroplet arising due to the difference in size of adjacent droplets.Artificial analogues of the natural nucleic acids have attracted interest as a diverse class of information storage molecules capable of self-replication. In this study, we use the computational potential energy landscape framework to investigate the structural and dynamical properties of xylo- and deoxyxylo-nucleic acids (XyNA and dXyNA), which are derived from their respective RNA and DNA analogues by inversion of a single chiral center in the sugar moiety of the nucleotides. For an octameric XyNA sequence and the analogue dXyNA, we observe facile conformational transitions between a left-handed helix, which is the free energy global minimum, and a ladder-type structure with approximately zero helicity. The competing ensembles are better separated in the dXyNA, making it a more suitable candidate for a molecular switch, whereas the XyNA exhibits additional flexibility. Both energy landscapes exhibit greater frustration than we observe in RNA or DNA, in agreement with the higher degree of optimization expected from the principle of minimal frustration in evolved biomolecules.Antimicrobial peptides are innate host defense molecules with the ability to kill pathogens. They have been widely studied for their membrane lytic activity and their potential to overcome the ever-increasing threat of antimicrobial resistance against conventional antibiotics. Here, we focus on two halictines, antimicrobial peptides first obtained from the venom of the eusocial bee Halictus sexcinctus. The peptides, HAL-1 and HAL-2, are cationic (with +3 and +4 charges, respectively) and amphipathic, have 12 amino acid residues, and exhibit high biological activity. For this study, the mechanism of action of HAL-1 and HAL-2 was studied in detail using large and giant unilamellar vesicles composed of pure palmitoyl oleoyl phosphatidyl choline (POPC) and a mixture of POPC and the anionic lipid palmitoyl oleoyl phosphatidyl glycerol (POPG) as biomimetic models of the membranes of eukaryotes and microorganisms, respectively. A set of complementary techniques was put forward carboxyfluorescein leakage assay, phase contrast optical microscopy, ζ-potential, static and dynamic light scattering, fluorescence and circular dichroism spectroscopies, and isothermal titration calorimetry.