The investigation of the variation of optoelectronic properties of molecules with changing patterns of nucleophilic substitution on 1,3,5-triazine core and presence of a hetero nitrogen atom in the donor part of the molecule is also accomplishedWe presented herien the rational design, synthesis, and photophysical property studies of the lysosome-targeted fluorescence FA probe NP-Lyso, an isopropyl group modified ortho-diaminonaphthalimide derivative After the reaction of FA and ortho-phenylenediamine modified with the isopropyl group in NP-Lyso, the probe exhibited favorable features such as a large fluorescence enhancement, specific selectivity and high sensitivity for the detection of FA More importantly, NP-Lyso could be used to detect and image endogenous FA in lysosomes In light of these prominent properties, we envision that NP-Lyso will be an efficient optical imaging approach for investigating the biofunctions of FA in living systemsSelenium containing drugs like selenomethionine, selenocystine, selenourea and methylseleninic acid are reported to exhibit potential anticancer effect However, these anticancer drugs may exert adverse effects when used over a prolonged period Little is known about the interaction of these selenium containing drugs with the vital erythroid protein hemoglobin In this work a comparative study of the interaction of organo-selenium drugs with hemoglobin and heme moiety has been performed using different spectroscopic techniques to find out their role on drug induced methemoglobinemia We found that though these selenium containing drugs have similar binding affinity towards hemoglobin, they have differential interactions with the heme group Isothermal calorimetric titration study showed that selenourea has the lowest binding affinity Kd 1928 μM towards HbA as compared to other drugs, selenomethionine, selenocystine and methylseleninic acid Kd 769 μM, 488 μM and 105 μM at 37 °C respectively This result is also supported by the molecular docking study Methylseleninic acid was found to have detrimental effects on nitrite induced methemoglobinemia, a hematological disorder caused due to excessive conversion of Fe2+ to Fe3+ in hemoglobin Hence the results of the study would help to develop a better insight on the mechanism of action and anticipate the toxicity of these drugs which require further optimization before their actual use in the treatment of cancerCombined antibiotic and heavy metal pollution has generated considerable concern Constructed wetlands CWs have been shown to efficiently remove pollutants; however, the microbial community responses to combined pollutants remain enigmatic In this study, seven microcosm CWs were planted with Myriophyllum aquaticum, spiked with tetracyclines TCs 300-30,000 μg/L, alone or with CuII, to investigate the response of plant-associated microbial communities TCs and the Cu/TC ratio greatly affected the performance of CWs Tetracyclines led to higher microbial diversity, evenness and richness, while UniFrac distances and principal coordinate PCO and redundancy analyses revealed that the co-presence of TCs and CuII led to variations in bacterial communities Proteobacteria, Cyanobacteria and Bacteroidetes were the dominant microbial phyla and Cloacibacterium, Hydrogenophaga, Rheinheimera and Denitratisoma accounted for 62-210 of all genera Therefore, the co-occurrence of heavy metals should be considered when judging the removal potential of TCs in phytoremediationThe retention of polyether sulfone PES and bisphenol A BPA in wastewater has received extensive attention The effects of PES and BPA on the removal of organic matter by anaerobic granular sludge were investigated We also analyzed the changes in the electron transport system and the effects on the composition of extracellular polymeric substances EPS, as well as alternations of the microbial community in the anaerobic granular sludge In the experimental groups which received BPA, the removal of the chemical oxygen demand COD were significantly suppressed, which an average removal efficiency of less than 65, 30 lower than that of the control group In the loosely-bound EPS LB-EPS excitation-emission matrix EEM spectra, the absorption peak of tryptophan disappeared when the BPA pollutants was added, which it was present in the control group without added pollutants The addition of PES and BPA also affected protease, acetate kinase, and coenzyme F420 activities in the anaerobic granular sludge Especially, the coenzyme F420 reduced from 00045 to 00017 μmol/L in the presence of PES and BPA The relative abundance of Spirochaetes decreased in the presence of PES and BPA, while the relative abundance of Bacteroidetes increased from 1298 to 2287 At the genus level, in the presence of PES and BPA, the relative abundance of Acinetobacter increased from 220 to 964 and Hydrogenophaga decreased sharply from 1558 to 012Antibiotic resistance genes ARGs are emerging micro-pollutants that pose potential threats to environments and humans Sewage sludge from wastewater is an important source for ARGs and current studies mainly focus on their existence in microbial genomes  https//wwwselleckchemcom/products/gbd-9html  However, little is known about which ARGs are expressed even though ARGs expression remains a better proxy for functional activity In this study, the expressed ARGs in sewage sludge were characterized by high-throughput quantitative PCR 296 primer sets combined with transcriptional analysis A total of 202 ARG transcripts were detected and their abundances ranged from 31 × 109 to 12 × 1010 copies/g dry weight The sum abundance of five most abundant ARG transcripts qacEdelta1-02, sul2, qacEdelta1-01, aadA2-03, tetX exhibited a linear correlation with the total abundance of ARG transcripts R2 = 088, p less then 10-4, suggesting that these genes could be regarded as indicators to quantitatively predict the total abundance of expressed ARGs Dynamics of expressed ARGs were observed with lower abundances in summer and winter than those in other seasons p less then 005, Kruskal-Wallis test Variation partitioning analysis indicated that the shift in bacterial community structures induced by changes in environmental attributes might be the main driver for the dynamics of expressed ARGs Results of this study provided new insights into the ARGs in sewage sludge