In conclusion, the MCP-1 A-2518 G polymorphism correlates with an elevated risk of AD and increased MCP-1 serum levels The interaction between the MCP-1 A-2518 G polymorphism and smoking contributes to the increased risk for AD in Chinese Han individualsHeroin use disorder is a chronic relapsing brain disease containing multiple phenotypes These phenotypes vary among heroin users and might be influenced by genetic factors Single-nucleotide polymorphisms SNPs of catechol-O-methyltransferase COMT and alpha-1-adrenergic receptor ADRA1A genes are associated with heroin use disorder However, it has not been clarified which phenotypes of heroin use disorder are related to these genes To address this question, we recruited 801 unrelated heroin users and divided them into different subgroups according to four important phenotypes of heroin use disorder Then 7 SNPs in the functional region of these genes were systematically screened and genotyped using a SNaPshot assay We found that the A allele of ADRA1A rs1048101 was associated with a shorter duration of transition from first use to addiction Subjects with the C allele of ADRA1A rs3808585 were more susceptible to memory impairment after heroin use disorder Subjects with the G allele of COMT rs769224 were more likely to take a higher dose of heroin every day Our study confirmed the association between polymorphisms of COMT and ADRA1A with those specific phenotypes of heroin use disorder, which will be instructive for the precise treatment of the disease
  Pseudomonas is a Gram-negative bacterial genus with numerous member species In this study, using whole-genome sequencing, we characterized a novel Pseudomonas sp  https//wwwselleckchemcom/screening/natural-product-libraryhtml  strain TUM18999, isolated as a pathogen from a human patient
 
  The TUM18999 strain was isolated from a patient's burn wound Minimum inhibitory concentrations MICs were determined using the broth microdilution method The whole-genome sequence was obtained using Miseq and MinION, and we conducted phylogenetic analysis based on single nucleotide polymorphisms of the core genome
 
  Antimicrobial susceptibility testing revealed a high ceftazidime MIC 32 mg/L Moreover, carbapenemase production was confirmed using the modified carbapenem inactivation method We found that the complete genome of TUM18999 was 6,826,062 bp long, with 6175 coding sequences CDS and a DNA G+C content non-plasmid of 664 mol Consistent with the high similarities with the 16S rRNA sequences of P otitidis MCC10330 986 and P alcaligenes NBRC 14159 992, similarities lt;90 were also observed with the gyrB genes of both strains The average nucleotide identities for P alcaligenes NBRC 14159 and P otitidis MCC10330 were also lt;90 The core-genome single nucleotide polymorphism phylogenetic tree indicated that the TUM18999 strain was most closely related to P otitidis MCC10330 In addition, the TUM18999 strain carried the novel gene, species-specific subclass B3 metallo-β-lactamase MBL, and its similarities with P alcaligenes metallo-β-lactamase-1 PAM-1 and P otitidis metallo-β-lactamase-1 POM-1 were 9024 and 7314, respectively
 
  We characterized the complete whole genome sequence of the novel Pseudomonas sp TUM18999 carrying the novel gene species-specific subclass B3 MBL
 We characterized the complete whole genome sequence of the novel Pseudomonas sp TUM18999 carrying the novel gene species-specific subclass B3 MBLWorking memory WM engagement produces pain inhibition However, it remains unclear whether higher WM load increases this effect The aim of this study was to investigate the interaction between WM load and pain inhibition by WM and examine the contribution of cerebrospinal mechanism Thirty-eight healthy volunteers were assigned to one of 2 n-back groups for which WM load was different 2-back or 3-back The experimental protocol comprised 5 counterbalanced conditions 0-back, n-back, pain, 0-back with pain, and n-back with pain Pain and the nociceptive flexion reflex NFR were evoked by transcutaneous electrical stimulation of the sural nerve Pain was significantly different between conditions, but not between n-back groups Both the 0-back and n-back tasks reduced pain compared with pain alone, but the n-back task produced stronger pain inhibition compared with the 0-back task NFR amplitude was significantly different between conditions but not between n-back groups NFR was inhibited by the 0-back and n-back tasks, with no difference between the 2 tasks These findings indicate that pain inhibition by WM is increased by WM load, but only to a certain point NFR inhibition by WM suggests that inhibition of pain by WM depends, at least in part, on cerebrospinal mechanism PERSPECTIVE This behavioral and electrophysiological study shows that engaging in a cognitive task reduces pain by decreasing spinal nociceptive transmission, depending on task difficulty These findings may yield better nonpharmacological pain therapies based on individual differences in working memory performance and capacity as well as several factors that regulate working memoryCopy number variants CNVs and gene mutations are important for diagnosis and treatment of myeloid malignancies In a routine clinical setting, somatic gene mutations are detected by targeted next-generation sequencing NGS assay, but CNVs are commonly detected by conventional chromosome analysis and fluorescence in situ hybridization FISH The aim of this proof-of-principle study was to investigate the feasibility of using targeted NGS to simultaneously detect both somatic mutations and CNVs Herein, we sequenced 406 consecutive patients with myeloid malignancies by targeted NGS and performed a head-to-head comparison with the results from a myelodysplastic syndrome MDS FISH and conventional chromosome analysis to detect CNVs Among 91 patients with abnormal MDS FISH results, the targeted NGS revealed all 120 CNVs detected by MDS FISH including -5/5q-, -7/7q-, +8, and 20q- and 193 extra CNVs detected by conventional chromosome analysis The targeted NGS achieved 100 concordance with the MDS FISH The lower limit of detection of MDS CNVs by the targeted NGS was generally 5 variant allele fraction for DNA, based on the lowest percentages of abnormal cells detected by MDS FISH in this study