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 The phenolic acids Sal A, Sal B, CA, and RA had been extracted from the chosen transgenic strains and quantified by comparison with normal curves as well as the corresponding retention instances Complete phenolic acids were being specified as TPA Anthocyanin contents were being determined by spectrophotometry by measuring the absorbance at 530 nm and 657 nm, with remedies without having extracts utilised as blank controls39
 
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 The recombinant vectors had been subsequently remodeled into yeast strain AH109, and also the autoactivation of pGKBT7-SmMYB1 was examined
 
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 A Expression styles of SmMYB1 in several tissues The fold improvements with the relative gene expression degree in all another tissues are normalized to the expression degree inside the calyx tissue B Effect of elicitors yeast extract, YE; abscisic acid, ABA; salicylic acid, SA; methyl jasmonate, MeJA; gibberellic acid, GA3; ethylene, Eth on SmMYB1 expression C Expression profiles of genes while in the phenolic acid biosynthesis pathway just after remedy with MeJA
 
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 appreciably decreased the expression of most genes with the phenylpropanoid pathway including PAL1 and C4H1 and also the tyrosine pathway like TAT1 and HPPR1, thereby inhibiting phenolic acid accumulation31 SmMYB39 negatively regulates the transcription and enzyme activities of C4H and TAT, thus decreasing rosmarinic acid contents, as shown in transgenic hairy roots overexpressing SmMYB3932 On  https//pastelinknet/7n3rkopr  , very few R2R3-MYB TFs that act as favourable regulators in selling phenolic acid accumulation are identified, as well as molecular system regulating phenolic acid biosynthesis induced by MeJA in S miltiorrhiza continues to be unknown
 
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